| Part I. Isolation and Culture of NSCs |
|
| 1. Neural Differentiation of Embryonic Stem Cells |
K. Sue O'Shea |
| 2. Production and Analysis of Neurospheres from Acutely Dissociated and Postmortem CNS Specimens |
Eric D. Laywell [and others] |
| 3. Isolation of Stem and Precursor Cells from Fetal Tissue |
Yuan Y. Wu, Tahmina Mujtaba, and Mahendra S. Rao |
| 4. Olfactory Ensheathing Cells : Isolation and Culture from the Rat Olfactory Bulb |
Susan C. Barnett and A. Jane Roskams |
| 5. Culturing Olfactory Ensheathing Glia from the Mouse Olfactory Epithelium |
Edmund Au and A. Jane Roskams |
| 6. Production of Immortalized Human Neural Crest Stem Cells |
Seung U. Kim [and others] |
| 7. Adult Rodent Spinal Cord Derived Neural Stem Cells : Isolation and Characterization |
Lamya S. Shihabuddin |
| 8. Preparation of Neural Progenitors from Bone Marrow and Umbilical Cord Blood |
Shijie Song and J. Sanchez-Ramos |
| 9. Seeding Neural Stem Cells on Scaffolds of PGA, PLA, and Their Copolymers |
Erin Lavik [and others] |
| Part II. Characterization of NSCs In Vitro |
|
| A. Cellular Techniques |
|
| 10. Analysis of Cell Generation in the Telencephalic Neuroepithelium |
Takao Takahashi, Verne S. Caviness, Jr., and Pradeep G. Bhide |
| 11. Clonal Analyses and Cryopreservation of Neural Stem Cell Cultures |
Angelo L. Vescovi, Rossella Galli, and Angela Gritti |
| 12. Assessing the Involvement of Telomerase in Stem Cell Biology |
Mark P. Mattson, Peisu Zhang, and Weiming Fu |
| 13. Detection of Telomerase Activity in Neural Cells |
Karen R. Prowse |
| 14. In Vitro Assays for Neural Stem Cell Differentiation |
Marcel M. Daadi |
| 15. Electron Microscopy and Lac-Z Labeling |
Bela Kosaras and Evan Snyder |
| B. Electrophysiological Techniques |
|
| 16. Techniques for Studying the Electrophysiology of Neurons Derived from Neural Stem/Progenitor Cells |
David S.K. Magnuson and Dante J. Morassutti |
| C. Molecular Techniques |
|
| 17. Fluorescence In Situ Hybridization |
Barbara A. Tate and Rachel L. Ostroff |
| 18. RT-PCR Analyses of Differential Gene Expression in ES-Derived Neural Stem Cells |
Theresa E. Gratsch |
| 19. Differential Display : Isolation of Novel Genes |
Theresa E. Gratsch |
| 20. Cell Labeling and Gene Misexpression by Electroporation |
Terence J. Van Raay and Michael R. Stark |
| 21. Gene Therapy Using Neural Stem Cells |
Luciano Conti and Elena Cattaneo |
| 22. Modeling Brain Pathologies Using Neural Stem Cells |
Simonetta Siplone and Elena Cattaneo |
| Part III. Utilization/Characterization of NSCs In Vivo |
|
| A. Endogenous Pools of Stem/Progenitor Cells |
|
| 23. Activation and Differentiation of Endogenous Neural Stem Cell Progeny in the Rat Parkinson Animal Model |
Marcel M. Daadi |
| 24. Identification of Musashil-Positive Cells in Human Normal and Neoplastic Neuroepithelial Tissues by Immunohistochemical Methods |
Yonehiro Kanemura, Shin-ichi Sakakibara, and Hideyuki Okano |
| 25. Identification of Newborn Cells by BrdU Labeling and Immunocytochemistry In Vivo |
|
| Sanjay S.P. Magavi and Jeffrey D. Macklis283 |
|
| 26. Immunocytochemical Analysis of Neuronal Differentiation |
Sanjay S.P. Magavi and Jeffrey D. Macklis |
| 27. Neuroanatomical Tracing of Neuronal Projections with Fluoro-Gold |
Lisa A. Catapano, Sanjay S.P. Magavi, and Jeffrey D. Macklis |
| B. Transplantation |
|
| 28. Labeling Stem Cells In Vitro for Identification of Their Differentiated Phenotypes After Grafting into the CNS |
Qi-lin Cao, Stephen M. Onifer, and Scott R. Whittemore |
| 29. Optimizing Stem Cell Grafting into the CNS |
Scott R. Whittemore [and others] |
| 30. Vision-Guided Technique for Cell Transplantation and Injection of Active Molecules into Rat and Mouse Embryos |
Lorenzo Magrassi |
| 31. Transplantation into Neonatal Rat Brain as a Tool to Study Properties of Stem Cells |
Tanja Zigova and Mary B. Newman |
| 32. Routes of Stem Cell Administration in the Adult Rodent |
Alison E. Willing [and others]. |
| Part I. Isolation and Culture of NSCs |
|
| 1. Neural Differentiation of Embryonic Stem Cells |
K. Sue O'Shea |
| 2. Production and Analysis of Neurospheres from Acutely Dissociated and Postmortem CNS Specimens |
Eric D. Laywell [and others] |
| 3. Isolation of Stem and Precursor Cells from Fetal Tissue |
Yuan Y. Wu, Tahmina Mujtaba, and Mahendra S. Rao |
| 4. Olfactory Ensheathing Cells : Isolation and Culture from the Rat Olfactory Bulb |
Susan C. Barnett and A. Jane Roskams |
| 5. Culturing Olfactory Ensheathing Glia from the Mouse Olfactory Epithelium |
Edmund Au and A. Jane Roskams |
