DNA topoisomerase protocols. Part 1, edited by Mary-Ann Bjornsti and Neil Osheroff

種類:

電子ブック

責任表示:

Osheroff, Neil. ; Bjornsti, Mary-Ann.

出版情報:

Totowa, N.J. : Humana Press, ©1999

著者名:

• Osheroff, Neil.
• Bjornsti, Mary-Ann.

シリーズ名:

Methods in molecular biology ; ; v. 94, pt. 1

ISBN:

9780585243993 [0585243999]    
9780896034440 [0896034445]    

注記:

DNA Topoisomerase Protocols, I: DNA Topology and Enzymes brings together an unprecedented collection of cutting-edge experimental protocols for investigating DNA structure, topology, and DNA topoisomerase function. Described by expert experimentalists who have perfected the techniques, these unfailingly reproducible methods utilize new approaches to study changes in DNA topology (linking number, knotting, catenation, and relaxation) and DNA structure (bending), as well as to assess chromosome structure through FLP-mediated recombination and to analyze bacterial nucleoid structure. State-of-the-art protocols also detail the expression and purification of bacterial, viral, and eukaryotic DNA topoisomerases from a variety of sources, including baculovirus, and bacterial, yeast and mammalian expression systems. A companion volume, DNA Topoisomerase Protocols, v. 2: Enzymology and Drugs, provides detailed protocols to study the catalytic activities of DNA topoisomerases, as well as their specific interactions with
Includes bibliographical references and index
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