Part I. General |
|
1. Fluorescent Measurement of [Ca2+]c : Basic Practical Considerations |
Alec W.M. Simpson |
2. Measurement of [Ca2+]i in Whole Cell Suspensions Using Fura-2 |
Robert A. Hirst [and others] |
3. Measurement of [Ca2+]i in Cell Suspensions Using lndo-1 |
Adriaan Nelemans |
Part II. Specialist Measurement Systems |
|
4. Confocal Microscopy : Theory and Applications |
Laura C. Mongan [and others] |
5. Measurement of Intracellular Calcium Concentration Using Confocal Microscopy |
Carmen Perez-Terzic, Marisa Jaconi, and Lisa Stehno-Bittel |
6. Single Cell and Subcellular Measurement of Intracellular Ca2+ Concentration ([Ca2+]i) |
Anthony J. Morgan and Andrew P. Thomas |
7. Measurement of [Ca2+] Using the Fluorometric Imaging Plate Reader (FLIPR) |
Elaine Sullivan, Emily M. Tucker, and Ian L. Dale |
Part III. Nonelectrophysiological Measurement of Ca2+ Channel Activity |
|
8. Measurement of Ca2+ Entry Using 45Ca2+ |
Mercedes Villaroya [and others] |
9. Measurement of [3H]PN200-110 and [125I]w-Conotoxin MVIIA Binding |
Kazuyoshi Hirota and David G. Lambert |
Part IV. Measurement of INS(1,4,5)P3 and Ca2+ Release from Intracellular Stores |
|
10. Measurement of Inositol (Poly)Phosphate Formation Using [3H]Inositol Labeling Protocols in Permeabilized Cells |
Philip Swigart and Shamshad Cockcroft |
11. Measurement of Inositol(1,4,5)Trisphosphate Using a Stereospecific Radioreceptor Mass Assay |
Darren Smart |
12. Measurement of Calcium Fluxes in Permeabilized Cells Using a45Ca2+ Uptake and Release Assay |
Robert A. Wilcox |
13. Microinjection of Myo-Inositol(1,4,5)Trisphosphate and Other Calcium-Mobilizing Agents into Intact Adherent Cells |
Robert A. Wilcox, Ian D. Forsythe, and Terence J. McCann |
14. Photolysis of Caged Calcium Using a Low-Cost Flash Unit |
Gommert A. van Koeveringe and Ron van Mastrigt |
15. Measurement of Ca2+ Flux through Ins(1,4,5)P3 Receptor-Ca2+ Channels in Lipid Bilayers ("Dip-Tip" and "Schindler" Methodology) |
Edwin C. Thrower |
16. Continuous Fluorescent Monitoring of Cellular Calcium Fluxes: A Novel Perfusion System for the Investigation of Inositol (1,4,5)Triphosphate-Dependent Quantal Calcium Release Using Immobilized, Electropermealized Cells |
Robert A. Wilcox and James Strupish |
17. Measurement of Free [Ca2+] Changes in Agonist-Sensitive Internal Stores Using Compartmentalized Fluorescent Indicators |
Aldebaran M. Hofer |
Part V. Specialized Measurement Techniques |
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18. Measurement of [Ca2+]i in Smooth Muscle Strips Using Front-Surface Fluorimetry |
Hideo Kanaide |
19. Measurement of Calcium and Movement in Heart Cells |
Leong L. Ng and Paulene A. Quinn |
20. Simultaneous Analysis of Intracellular pH and Ca2+ from Cell Populations |
Paul Martinez-Zaguilan [and others] |
21. Measurement of Cytosolic-Free Ca2+ in Plant Tissue |
Martin R. McAinsh and Irina Staxen |
Part VI. Ca2+ Sensitive Targets |
|
22. Assay and Purification of Calmodulin-Dependent Protein Kinase |
Rakesh Kakkar and Rajendra K. Sharma |
23. Measurement of Ca2+-ATPase Activity (in PMCA and SERCA1) |
Danuta Kosk-Kosicka. |
Part I. General |
|
1. Fluorescent Measurement of [Ca2+]c : Basic Practical Considerations |
Alec W.M. Simpson |
2. Measurement of [Ca2+]i in Whole Cell Suspensions Using Fura-2 |
Robert A. Hirst [and others] |
3. Measurement of [Ca2+]i in Cell Suspensions Using lndo-1 |
Adriaan Nelemans |
Part II. Specialist Measurement Systems |
|
4. Confocal Microscopy : Theory and Applications |
Laura C. Mongan [and others] |